Tissue-specific DNA damage response in Mouse Whole-body irradiation

Abstract Background Genomic instability is a hallmark of various cancers, and DNA repair an essential process for maintaining genomic integrity. Mammalian cells have developed mechanisms in response to damage. Compared the cellular damage, vivo damage (DDR) specific tissues has not been studied extensively. Objective In this study, mice were exposed whole-body gamma (γ)-irradiation evaluate DDR tissues. We treated male C57BL6/J with γ-irradiation at different doses, protein levels analyzed. Results The level gamma-H2A histone family member X (γH2AX) increased most organs after exposure γ-irradiation. particular, liver, lung, kidney showed higher γH2AX induction upon compared that brain, muscle, testis RAD51 was highly expressed testis, irrespective irradiation. proliferating cell nuclear antigen (PCNA) ubiquitinated PCNA lung irradiation, suggesting post-replication may mainly operate lungs Conclusion These results suggest each tissue preferable mechanism Therefore, understanding application tissue-specific responses could improve clinical approach radiotherapy treating cancers.